A biocomplex to repair experimental critical size defects associated with photobiomodulation therapy

Abstract Background: The association of scaffolds to repair extensive bone defects can contribute to their evolution and morphophysiological recomposition. The incorporation of particulate biomaterials into three-dimensional fibrin bioproducts together with photobiomodulation therapy (PBM) has potential and can improve regenerative medicine procedures. The objective of this experiment was to evaluate the effects of PBM therapy on critical size defects filled with xenogenic bone substitute associated with fibrin biopolymer. Methods: A critical defect of 8 mm was performed in 36 Wistar male adult rats that were divided into four groups. Groups BC and BC-PBM were defined as controls with defects filled by a clot (without or with PBM, respectively) and groups XS and XS-PBM that comprised those filled with biocomplex Bio-OssTM in association with fibrin biopolymer. PBM was applied immediately after the surgery and three times a week every other day, with the parameters: wavelength of 830 nm, energy density 6.2 J/cm2, output power 30 mW, beam area of 0.116 cm2, irradiance 0.258,62 W/cm2, energy/point 0.72 J, total energy 2.88 J. Fourteen and 42 days after the surgery, animals were euthanatized and subjected to microtomography, qualitative and quantitative histological analysis. Results: The BC-PBM and XS-PBM groups had a similar evolution in the tissue repair process, with a higher density of the volume of new formed bone in relation to the groups without PBM (p = 0.04086; p = 0.07093, respectively). Intense vascular proliferation and bone deposition around the biomaterial particles were observed in the animals of the groups in which biocomplex was applied (XS and XS-PBM). Conclusion: PBM therapy allowed an improvement in the formation of new bone, with a more organized deposition of collagen fibers in the defect area. Biocomplex favored the insertion and permanence of the particulate material in bone defects, creating a favorable microenvironment for accelerate repair process.

A biocomplex to repair experimental critical size defects associated with photobiomodulation therapy

The association of scaffolds to repair extensive bone defects can contribute to their evolution and morphophysiological recomposition. The incorporation of particulate biomaterials into three-dimensional fibrin bioproducts together with photobiomodulation therapy (PBM) has potential and can improve regenerative medicine procedures. The objective of this experiment was to evaluate the effects of PBM therapy on critical size defects filled with xenogenic bone substitute associated with fibrin biopolymer. Methods: A critical defect of 8 mm was performed in 36 Wistar male adult rats that were divided into four groups. Groups BC and BC-PBM were defined as controls with defects filled by a clot (without or with PBM, respectively) and groups XS and XS-PBM that comprised those filled with biocomplex Bio-OssTM in association with fibrin biopolymer. PBM was applied immediately after the surgery and three times a week every other day, with the parameters: wavelength of 830 nm, energy density 6.2 J/cm2, output power 30 mW, beam area of 0.116 cm2, irradiance 0.258,62 W/cm2, energy/point 0.72 J, total energy 2.88 J. Fourteen and 42 days after the surgery, animals were euthanatized and subjected to microtomography, qualitative and quantitative histological analysis. Results: The BC-PBM and XS-PBM groups had a similar evolution in the tissue repair process, with a higher density of the volume of new formed bone in relation to the groups without PBM (p = 0.04086; p = 0.07093, respectively). Intense vascular proliferation and bone deposition around the biomaterial particles were observed in the animals of the groups in which biocomplex was applied (XS and XS-PBM). Conclusion: PBM therapy allowed an improvement in the formation of new bone, with a more organized deposition of collagen fibers in the defect area. Biocomplex favored the insertion and permanence of the particulate material in bone defects, creating a favorable microenvironment for accelerate repair process.(AU)

Reproduction of Esthetic Individuality in Upper Immediate Complete Denture / Reproducción de la Individualidad Estética en Prótesis Completa Inmediata Superior

ABSTRACT: Prosthodontics, in general, aims to rehabilitate the masticatory function of the patient, as well as the stomatognathic system, maintaining his or her individual facial characteristics. The immediate removable complete denture is placed immediately after extraction of the natural teeth and allows adaptation of the patient from the dentate state to the denture, until the definitive denture is placed. When an immediate complete denture is fabricated, esthetics plays a fundamental role and thus the assembly of artificial teeth can be performed maintaining the same position, alignment and arrangement of the remaining anterior teeth, providing a natural and esthetic appearance to the denture, thus the transition from the dentate to the edentulous state is less noticeable. This paper reports the case of a patient who needed oral rehabilitation with an immediate upper complete denture and presented favorable smile esthetics of the anterior teeth, which allowed the preservation of alignment, position and arrangement of natural teeth during the assembly of artificial teeth, maintaining and preserving the esthetic individuality and facial harmonization, meeting the patient's desire and expectations.

RESUMEN: La prostodoncia, en general, tiene como objetivo rehabilitar la función masticatoria del paciente, así como el sistema estomatognático, manteniendo sus características faciales individuales. La dentadura postiza completa removible se coloca inmediatamente después de la extracción de los dientes naturales y permite la adaptación del paciente del estado dentado a la dentadura, hasta que se coloque la dentadura definitiva. Cuando se fabrica una dentadura postiza completa inmediata, la estética juega un papel fundamental y, por lo tanto, el ensamblaje de dientes artificiales se puede realizar manteniendo la misma posición, alineación y disposición de los dientes anteriores restantes, proporcionando un aspecto natural y estético a la dentadura, por lo tanto, la transición desde el estado dentado hasta el estado desdentado es menos notable. Este artículo informa el caso de una paciente que necesitó rehabilitación oral con una dentadura postiza completa superior inmediata y presentó una estética de sonrisa favorable de los dientes anteriores, lo que permitió preservar la alineación, la posición y la disposición de los dientes naturales durante el ensamblaje de los dientes artificiales, manteniendo y preservando la individualidad estética y la armonización facial, satisfaciendo los deseos y expectativas del paciente.

Unique heterologous fibrin biopolymer with hemostatic, adhesive, sealant, scaffold and drug delivery properties: a systematic review

Fibrin biopolymers, previously referred as "fibrin glue" or "fibrin sealants", are natural biomaterials with diverse applications on health. They have hemostatic, adhesive, sealant, scaffold and drug delivery properties and have become widely used in medical and dental procedures. Historically, these biomaterials are produced from human fibrinogen and human or animal thrombin, and the possibility of transmission of infectious diseases by human blood is not ruled out. In the 1990s, to overcome this problem, a new heterologous biomaterial composed of a thrombin-like enzyme purified from Crotalus durissus terrificus venom and a cryoprecipitate rich in fibrinogen extracted from buffaloes Bubalus bubalis blood has been proposed. Therefore, a systematic review of studies on exclusively heterologous fibrin sealants published between 1989 and 2018 was carried out using the following databases: PubMed, SciELO and Google Scholar. The keyword used was "heterologous fibrin sealant". The search resulted in 35 scientific papers in PubMed, four in SciELO and 674 in Google Scholar. After applying the inclusion/exclusion criteria and complete reading of the articles, 30 studies were selected, which formed the basis of this systematic review. It has been observed that the only completely heterologous sealant is the one produced by CEVAP/UNESP. This heterologous biopolymer is proven effective by several studies published in refereed scientific journals. In addition, clinical trials phase I/II for the treatment of chronic venous ulcers authorized by the Brazilian Health Regulatory Agency (ANVISA) were completed. Preliminary results have indicated a safe and promising effective product. Phase III clinical trials will be proposed and required to validate these preliminary findings.(AU)

Action of a deproteinized xenogenic biomaterial in the process of bone repair in rats submitted to inhalation of cigarette smoke

Abstract Purpose: To investigate if the inorganic bovine bone matrix changes the bone formation in rats submitted to inhalation of cigarette smoke. Methods: Twenty Wistar rats were divided into two groups: Cigarette Clot Group (CCG), which in the inhalation chamber received the smoke of 10 cigarettes, 3 times a day, 10 minutes, for 30 days and had the surgical cavity filled by clot; Cigarette Biomaterial Group (CBG), submitted to the same inhalation technique but with the cavity filled by biomaterial. Results: In CCG there was a significant difference of new bone tissue in the analyzed periods (15 and 45 days), and in 15 days, there was 4.8 ± 0.42 of bone formed and 11.73 ± 0.59 (p <0.05) in 45 days. The CBG also showed a significant difference between the periods of 15 to 45 days, being respectively 6.16 ± 0.30 and 11.60 ± 0.61. However, when the groups were compared, within the same analyzed periods, a significant difference was observed only in the period of 15 days, with the new bone percentage being greater in the CBG. Conclusion: The bone matrix acted as an osteoinductive biomaterial, biocompatible and aided in the repair process, mainly in the initial period of recovery.

Inside-out and standard vein grafts associated with platelet-rich plasma (PRP) in sciatic nerve repair. A histomorphometric study

Abstract Purpose: To evaluated the tubulization technique with standard and inside-out vein, filled or not with platelet-rich plasma (PRP), in sciatic nerve repair. Methods: Seventy male Wistar rats were randomly divided into five groups: IOVNF (Inside-Out Vein with No Filling); IOVPRP (Inside-Out Vein filled with PRP); SVNF (Standard Vein with No Filling); SVPRP (Standard Vein filled with PRP); Sham (Control). The left external jugular vein was used as graft in a 10 mm nervous gap. Results: In the morphological analysis of all groups, myelinated nerve fibers with evident myelin sheath, neoformation of the epineurium and perineurium, organization of intraneural fascicles and blood vessels were observed. In the morphometry of the distal stump fibers, SVPRP group had the highest means regarding fiber diameter (3.63±0.42 μm), axon diameter (2.37±0.31 μm) and myelin sheath area (11.70±0.84 μm2). IOVPRP group had the highest means regarding axon area (4.39±1.16 μm2) and myelin sheath thickness (0.80±0.19 μm). As for values of the fiber area, IOVNF group shows highest means (15.54±0.67 μm2), but are still lower than the values of the Sham group. Conclusion: The graft filled with platelet-rich plasma, with use standard (SVPRP) or inside-out vein (IOVPRP), promoted the improvement in axonal regeneration on sciatic nerve injury.

Multidisciplinary approach in the teaching of dental sculpture and anatomy / Enfoque multidisciplinario para la enseñanza de escultura y anatomía dental

The purpose of the Dental Sculpture and Anatomy discipline is to introduce undergraduate students to the study of the anatomic and morphological characteristics of permanent and primary human dentition, through classes, books and cognitive and psychomotor activities. This discipline supports the teaching of specific knowledge necessary for a more extensive education, involving interdisciplinarity as a means of knowledge exchange among several areas of dentistry, to achieve comprehensive professional education. Students must recognize the dental morphology from samples of preserved teeth, and reproduce the morphology through three-dimensional models made of stone or wax blocks. In this article, the authors describe the process for producing teeth collars and macro dental models made of stone, their importance and benefits of utilization. The purpose of the study was to encourage the teaching of Dental Sculpture and Anatomy toundergraduate students of the Bauru School of Dentistry, University of São Paulo, through activities that would associate theory, practice and the development of manual skills.

El propósito de la disciplina sobre escultura y anatomía de dientes es introducir a los estudiantes de pregrado para el estudio de las características anatómicas y morfológicas de la dentición humana permanente y primaria, a través de clases, libros y actividades cognitivas y psicomotoras. Esta disciplina se fundamenta en la enseñanza de conocimientos específicos necesarios para una educación más amplia, que implica la interdisciplinariedad como medio de intercambio de conocimientos entre diferentes áreas en odontología, y de esa forma, lograr una enseñanza profesional integral. Los estudiantes deben identificar la morfología dental a partir de dientes conservados, además de reproducir la morfología a través de modelos tridimensionales elaborados con bloques de yeso tipo piedra o cera. En este estudio, los autores describen el proceso para la producción de collares de dientes y macro modelos dentales hechos de yeso tipo piedra, su importancia y los beneficios de su utilización. El propósito del estudio fue incentivar la enseñanza de la escultura y anatomía dental a los estudiantes de pregrado de la Facultad de Odontología de Bauru, Universidad de São Paulo, a través de actividades que asocian la teoría, práctica y desarrollo de habilidades manuales.

Reparo do nervo facial com sutura epineural térmico-terminal e coaptação com adesivo de fibrina em ratos associado ou não a laserterapia / Repair of facial nerve with epineural end-to-end suture and coaptation with fibrin adhesive in rats associated or not laser therapy

As lesões que envolvem nervos periféricos, especialmente os traumatismos facias, são muito comuns e decorrentes principalmente de acidentes com veículos motorizados, lesões acidentais e quedas, que levam a fraturas do osso temporal ou lacerações da face e consequentemente lesões do nervo facial. A principal meta no estudo da regeneração nervosa é descobrir uma técnica adequada de reparo em lesões de nervos periféricos que traga como resultado a recuperação funcional das estruturas por eles inervadas. A sutura epineural é um método muito utilizado para recuperação de lesões nervosas, assim como o uso do adesivo de fibrina, que requer menor destreza do cirurgião. O adesivo derivado do veneno de serpente (CEVAP/UNESP, Botucatu-SP) é um selante biológico e biodegradável, pois não produz reações adversas, não contém sangue humano, apresenta uma boa capacidade adesiva, não transmite doenças infecciosas, e pode ser utilizado como coadjuvante em procedimentos de sutura convencional. Sendo assim, o objetivo deste trabalho foi comparar duas técnicas de recuperação de nervos periféricos lesionados: a sutura epineural término-terminal e o adesivo de fibrina derivado do veneno de serpente, e observar se o uso da laserterapia de baixa potência influencia esse processo de regeneração. Para isso, foram utilizados 42 ratos machos (Rattus norvegicus, Wistar), com 60 dias de vida, separados aleatoriamente em um Grupo Controle e quatro Grupos Experimentais, assim formados: Grupo Controle (GC, n=10), em que foi coletado o nervo facial íntegro aos 95 e 135 dias de vida; Grupo Experimental Sutura (GES, n=16) e Grupo Experimental Adesivo de Fibrina (GEF, n=16), onde no lado direito da face o ramo bucal do nervo facial foi seccionado e realizado a sutura epineural término-terminal e, no lado esquerdo da face, o ramo bucal do nervo facial foi seccionado e utilizado o adesivo de fibrina para coaptação das extremidades; Grupo Experimental Sutura e Laserterapia (GESL, n=16)...

The injuries involving peripheral nerves, especially facial traumatisms are very common and serious and longstanding facial paralysis lead to significant deterioration in the quality of the individuals life. The main goal in the study of nerve regeneration is finding a suitable repair technique for peripheral nerve injuries that bring results in the functional recovery of the structures innervated by them. Therefore, the aim of this study was to compare two techniques for recovery of injured peripheral nerves: the end-to-end epineural suture and fibrin adhesive derived from snake venom (CEVAP / UNESP, Botucatu-SP), and observeif the use of low-level laser therapy influences this regeneration process. For this purpose, 42 male rats (Rattus norvegicus, Wistar) were used , with 60 days of life, were randomly separated into a control group and four experimental groups, which were formed this way: Control Group (CG , n = 10), in which the intact facial nerve was collected at 95 and 135 days of life; Experimental Suture Group (ESG, n = 16 ) and Experimental Fibrin Adhesive Group (EFG, n = 16), where the right side of the face the buccal branch of the facial nerve was transectioned and the epineural end-to-end suture was performed and the left side of the face, the buccal branch of the facial nerve was transectioned and the fibrin glue was used for coaptation of the edges; Experimental Suture Laser Therapy Group (ESLG, n = 16) and Experimental Fibrin Adhesive Laser Therapy Group (EFLG, n = 16) underwent the same procedures of ESG and EFG , included the application of Laser Gallium- Aluminum-Arsenide (GaAlAs) by an 830 nm wavelength pulse continuous, 6 J/cm2, for 24 seconds, three times a week during five weeks at three points of the operated areas. The animals in the experimental groups were euthanized at 95 days (five weeks post-surgery) and 135 days (ten weeks post-surgery). The collected samples were...

Ozone Therapy in Dentistry: A Systematic Review / Ozonoterapia en Odontología: Una Revisión Sistemática

The purpose of this systematic review was to verify the literature available regarding the effectiveness and the biological effects of ozone therapy in periodontics, orthodontics and dental implants. Studies were searched in September 2012. Analyzed sources included the databases PubMed, Lilacs and SciELO, through a combination of key words, dental implants, periodontics, orthodontics, therapeutics. Studies between 2002 and 2012 were included. In vitro and in vivo studies English and Spanish language publications, excluding posters, letters to the editors and conferences. In Vitro and in Vivo studies showed the inactivation of the major periodontal pathogens by ozone. There were divergent results and lack of evidence for the activity of ozone on adhesion of orthodontic brackets, in implantology and orthodontics. Ozone could be considered as a promissory alternative therapy in dentistry. However, well-designed studies are needed to assess the application of ozone in order to evaluate its clinical effectiveness in the field of dentistry.

El objetivo de esta revisión sistemática fue verificar la literatura disponible relacionada a la efectividad y efectos biológicos de la ozonoterapia en periodoncia, ortodoncia e implantes dentales. Se realizó una revisión sistemática en septiembre del 2012 en las bases de datos PubMed, LILACS and SciELO por medio de la combinación de las palabras clave dental implants, periodontics, orthodontics, therapeutics. Se evaluaron los artículos publicados entre los años 2002 y 2012. Fueron incluidos estudios In vitro e in vivo y publicaciones en Inglés y Español, excluyendo pósters, carta al editor y conferencias. Estudios in vitro e in vivo mostraron inactivación de los principales patógenos periodontais con el uso del ozono. Hubo resultados contradictorios y falta de evidencia sobre la acción del ozono en la adhesión de los brackets ortodónticos, así como también en la osteointegración de los implantes dentales. El ozono puede ser considerado como una terapia alternativa promisoria en la Odontología. Sin embargo, es necesaria la realización de estudios bien diseñados para, de ese modo, evaluar la aplicación del ozono y su efectividad clínica en el campo de la Odontología.

Anatomical, clinical and radiographic characteristics of styloid syndrome (Eagle syndrome): a case report / Características anatómicas, clínicas y radiográficas del síndrome Estiloide (síndrome de Eagle): un caso clínico

Styloid syndrome is characterized by an elongated styloid process or calcification of the stylomandibular and stylohyoid ligament. This study describes a case of a 65-year-old woman who presented to the Stomatology Clinic, University of Marilia with temporomandibular joint pain, ear ringing and a reduced ability to open the mouth. Panoramic and posteroanterior digital radiographs showed bilateral elongation of the styloid processes, especially of the right side, whose length extended beyond the mandible angle. The diagnosis was confirmed with the association of clinical data and image examinations. The treatment options for styloid Syndrome include clinical follow-up, surgical removal of the styloid process or fracture of the elongated process. The case was managed by providing prosthetic rehabilitation and clinical follow-up, observing the level of discomfort and the benefit that could be achieved by the therapy, and avoiding surgical intervention.

El síndrome estilode (Síndrome de Eagle) se caracteriza por un proceso estiloides alargado o calcificación de los ligamentos estilohioideo y estilomandibular. Este estudio describe el caso de una mujer de 65 años de edad que concurrió a la Clínica Estomatológica de la Universidad de Marilia con dolor de la articulación temporomandibular, zumbido en los oídos y una disminución de la capacidad de abrir la boca. Las radiografías digitales panorámica y posteroanterior mostraron alargamiento bilateral de los procesos estiloides, especialmente del lado derecho, cuya longitud se extendió más allá del ángulo de la mandíbula. El diagnóstico se confirmó con la asociación de los datos clínicos y los exámenes de imagen. Las opciones de tratamiento para el síndrome estiloide incluyen el seguimiento clínico, la extirpación quirúrgica del proceso estiloides o la fractura del proceso alargado. El caso fue tratado con rehabilitación protésica y seguimiento clínico, observando el nivel de molestia y el beneficio que se podría lograr mediante la terapia, evitando la intervención quirúrgica.

Bovine bone matrix action associated with morphogenetic protein in bone defects in rats submitted to alcoholism / Acción de la matriz ósea bovina asociada a la proteína morfogenéticaen defectos óseos en ratones sometidos a alcoholismo

Extended excessive alcohol use causes changes in bone tissue, thus affecting osteogenesis. The objective of this study was to evaluate if demineralized bone matrix (Gen-ox®) associated with bone morphogenetic protein (Gen-pro®) changes bone neoformation in rats submitted to experimental alcoholism. Forty male rats (Rattus norvegicus) were separated into 2 groups of 20 animals each: Group E1, which received ethyl alcohol at 25 percent and had the surgical cavity filled in only with blood clot; and Group E2, which received ethyl alcohol at 25 percent and had the surgical cavity filled in with demineralized bovine cortical bone associated with bone morphogenetic protein. The animals were submitted to a three-week period of gradual adaptation to alcohol, and then continued receiving alcohol at 25 percent for 90 days, when the surgical cavity was made. After the surgery, the animals continued consuming alcohol until reaching the sacrifice periods of 10, 20, 40, and 60 days, when the tibias were removed for histological processing. Results showed that surgical cavity repair and bone marrow reorganization occurred faster in Group E1 than in Group E2. At the end of the experiment, it was observed that animals in Group E2 had thick bony trabeculae surrounding the implanted material particles and a small area of connective tissue in the surface region. In conclusion, the implanted material did not accelerate bone neoformation, rather it served as a structure for osteogenesis.

El abuso prolongado del alcohol produce alteraciones en el tejido óseo, interfiriendo en el proceso de la osteogénesis. El estudio tiene como objetivo evaluar si la matriz ósea bovina desmineralizada (Gen-ox®) asociada a la proteína morfogenética ósea (Gen-pro®) altera la neoformación ósea en ratones sometidos a alcoholismo experimental. Fueron utilizados 40 ratones machos (Rattus norvegicus), separados en dos grupos de 20 animales cada uno: Grupo E1, que recibió alcohol etílico a 25 por ciento con cavidad quirúrgica rellenada solamente por coágulo sanguíneo, y Grupo E2, que recibió sólo alcohol etílico a 25 por ciento con cavidad quirúrgica rellenada con hueso bovino desmineralizado cortical asociado a proteína morfogenética ósea. Después de 3 semanas de adaptación gradual al alcohol, los animales continuaron recibiéndolo en concentración de 25 por ciento por 90 días, cuando fue realizada la cavidad quirúrgica. Luego de la cirugía, los animales continuaron la ingestión alcohólica hasta los períodos de sacrificio de 10, 20, 40 y 60 días, cuando las tibias fueron removidas para su procesamiento histológico. Los resultados mostraron que en el Grupo E1 hubo reparación de la cavidad quirúrgica y reorganización de la médula ósea en un menor lapso temporal que en el Grupo E2. En el período final del experimento, se observó en los animales del Grupo E2 la presencia de trabéculas óseas espesas alrededor de las partículas de material implantado y pequeña área de tejido conjuntivo en la región superficial. Se puede concluir en que el material implantado no aceleró el proceso de neoformación ósea, sirviendo como estructura de base para generar osteogénesis.

Efeitos de 03 dietas alcoólicas na reparação óssea em tíbia de ratos / Effects of three alcoholic diets on the bone repair in the tibia of rats

Os efeitos do uso abusivo e prolongado do álcool sobre vários tecidos, incluindo o ósseo, têm sido amplamente estudados. A quantidade de defeitos ósseos criados cirurgicamente e pelos vários tipos de acidentes tem aumentado e existe uma preocupação muito grande quanto ao reparo desses defeitos, associados ao alcoolismo. O objetivo desta pesquisa foi de observar histologicamente os efeitos de 03 dietas alcoólicas na reparação óssea e se o aumento da concentração do álcool também interfere neste fenômeno. Foram utilizados 64 ratos machos (Rattus norvegicus), divididos em 4 grupos de 16 animais cada, assim distribuídos: Grupo controle (C) – animais que não receberam álcool; Grupos Experimentais I, II e III (EI, EII e EIII, respectivamente) - animais que foram submetidos à alcoolização de 6%, 15% e 25% respectivamente, por um período de 90 dias. Após este período a tíbia esquerda de todos os animais foi submetida a uma cirurgia onde se realizou uma cavidade no sentido longitudinal. Quatro animais de cada grupo foram sacrificados em períodos de 10, 20, 40 e 60 dias contados a partir do dia da cirurgia experimental. As tíbias foram retiradas, processadas histologicamente e submetidas às colorações de HE e tricrômico de Masson. Os resultados mostraram que a neoformação óssea foi decrescente de acordo com o aumento da concentração alcoólica e pode-se concluir que as 03 dietas alcoólicas (6%, 15% e 25%) influenciam a neoformação óssea em todas as suas fases, retardando o processo de reparação óssea.

The effects of long-term alcohol abuse on several tissues, including bone, have been extensively studied. Considering the increase in occurrence of bone defects caused by trauma or even created for surgical purposes, the putative influence of alcoholism on bone healing has been a matter of growing concern. The aim of the present study was to assess histologically the effects of three schemes of alcohol intake at different concentrations on bone repair. 64 male rats (Rattus norvegicus) were assigned to four experimental groups (n=16), which received alcohol diluted with drinking water to the final concentrations of 6% (Group EI), 15% (Group EII) or 25% (Group EIII). The control group (Group C) received drinking water. After 90 days, the animals were subjected to a surgical procedure in which a cavity (3 mm in width) was created on the left tibia. Four animals of each group were sacrificed on 10th, 20th, 40th or 60th days after the surgical procedure. The samples were processed for histological analysis, paraffin embedded and stained with hematoxylin and eosin; and Masson tricromic. The qualitative histological analysis showed that the amount of bone neoformation was inversely proportional to the alcohol concentration. It may be concluded that the schemes of alcohol consumption at the concentrations used in the present study (6%, 15% e 25%) led to a decrease in osseous neoformation in all analyzed periods, resulting in delay on the healing process.

Biocompatibility of anionic collagen matrices and its influence on the orientation of cellular growth / Biocompatibilidade de matrizes de colágeno aniônico e sua influência na orientação do crescimento celular

This study aimed to analyze the biocompatibility of anionic collagen matrices, the local bone response following implantation in surgically-created bone defects, and also the influence of the collagen fiber orientation on the neoformed osseous tissue. Seventy two rats (Rattus norvegicus albinus) were used in this experiment. The animals were divided into four experimental groups: Group 1 (control), without implants; Group 2, pericardium medullar implants 12 hours; Group 3, medullar implants of tendon 24 hours, with the long axis of collagen fibers oriented parallel to the long axis of the tibia; and Group 4, medullar implants of tendon 24 hours, with the long axis of collagen fibers perpendicular to the long axis of the tibia. After the experimental surgery, the evolution of the repair process was microscopically evaluated at 7, 15, and 30 days post-surgery. The results demonstrated that the implanted matrices are biocompatible and act as a scaffold inducing bone formation, mainly in the Group 4 animals. At first, cellularity follows the arrangement of collagen fibers, later obtaining a multidirectional growth.

Este estudo apresentou como objetivo, examinar a biocompatibilidade de matrizes de colágeno aniônico, sua respostaóssea local após implantação em defeitos ósseos criados cirurgicamente e a influência da orientação das fibras colágenasno tecido ósseo neoformado. Foram utilizados 72 ratos (Rattus norvegicus albinus) divididos em 4 grupos experimentaisde 6 animais cada: Grupo 1 (Controle), sem implante; Grupo 2, implante medular de pericárdio 12 horas; Grupo 3,implante medular de tendão 24 horas, com o maior eixo de suas fibras colágenas direcionados paralelamente ao maioreixo da tíbia e, Grupo 4, implante medular de tendão 24 horas, com o maior eixo de suas fibras colágenas direcionados perpendicularmente ao maior eixo da tíbia. A evolução do processo de reparo, após a cirurgia experimental, foi avaliada microscopicamente com 7, 15 e 30 dias pós-operatórios. Os resultados demonstraram que as matrizes implantadas são biocompatíveis e funcionam como uma matriz tridimensional induzindo a formação de osso, maior no grupo 4. A celularidade, inicialmente, acompanha o arranjo das fibras colágenas, adquirindo um arranjo multidirecional crescente.